Main Article Content
Scoparia dulcis, Antibacterial activity, Antilithiatic activity, Calcium oxalate dissolution method, Gas chromatography, mass spectrometry,
Scoparia dulcis Linn has been widely reported to have pharmacological uses arising from its wide spread uses. The different extracts were prepared by successive extraction with petroleum ether, chloroform, ethanol and water using soxhlet distillation method. Phytochemical analysis of plant extract revealed the presence of alkaloids, carbohydrates, glycoside, tannins, starch etc.
Thin layer chromatography and Gas chromatography-mass spectrometric analysis revealed the presence of different components in the plant extract. Among the 50 components obtained, 10 important organic compounds were analyzed. All these compounds are found to be having some medicinal application.
UV visible spectroscopic analysis of extract of Scoparia dulcis reported four chromatogram figures which showed prominent peaks having maximum absorption of 666 nm corresponded to wavelength of methylene blue and brilliant blue. FTIR spectroscopic analysis reveals the presence of important functional groups like-OH,-NO2,-SO3,-SH, -COOH, NH2, R-X etc. Antibacterial activity of petroleum ether, chloroform, and ethanol and water extracts of stems and leaves of Scoparia dulcis reveals that chloroform and ethanol extracts shows maximum resistance against Staphylococcus while ethanol and aqueous extracts showed maximum resistance against Klebsiela pneumonia.
The exciting fact came out of the study is that water extract of Scoparia dulcis showed great potential to dissolve the Calcium oxalate crystals ie, the plant extract shows invitro antilithiatic activity for kidney stones. Thus Scoparia dulcis act as a source of different valuable organic compounds that are having medicinal applications and have a beneficial effect on kidney stone problem.
2. Prachi Khare, Vinod Kumar Mishra, Kakkar Arun, Neethu Bais. “Study on invitro antilithiatic activity of Phyllanthus Narre Linn leaves by homogeneous precipitation and turbidity method”. International Journal of pharmacy and pharmaceutical Sciences 6(4) 2014.
3. Rasool Hassan, “Pharmaceut, Anal Acta” 3(10) 2012.
4. Tapsell L C, Hemphill J, Coboac L, etal. “Health benefits of herbs and spices- the past, the present, and the future”. Med.J. Aust.185 (4 suppl):S4-24. PMIDI7022438, August 2006.
5. Sherman P, Hash GA “Why vegetable recipes are not very spicy” Evol Hum Behav. 22(3):147-163. P MID 11384883 May, 2001.
6. Angiosperms: Division Magnoliophyta: General Features “Encyclopedia Britannica 13 15th edition) P 609, 1993.
7. Okhale, Samuel Ehiabhi, Amanado Mercy Oma Jegede. “Phytochemical and pharmacognostic investigation of Anti diabetic Scoparia dulcis Linn. Scrophularicea whole plant Grown in Nigeria. Researcher 2(6) 2010.
8. Prachi Khare, Vinod Kumar Mishra, Kakkar Arun Neethu Bais, Rajendra Singh, “Study on invitro antilithiatic activity of Phyllanthees Niruri Linn leaves by homogeneous precipitation and turbidity method. International Journal of pharmacy and pharmaceutical sciences”6(4), 2014.
9. Stepp, John R. “The role of weeds as sources pharmaceuticals” Journal of ethanopharmacology 92(2-3): 163-166. PMID 15137997 June 2004.
10. Stepp, John R and Moerman, Daniel E (April “The importance of weeds in ethno pharmacology” Journal of Ethnophramacology 75 (1) :19-23 June, 2004.
11. Sameer, Judith. The Natural History of Medicinal plants. Timber press. P 16 ISBN 0-88192, 483, 2000.
12. Grish Diwedi, Shridhar Divedi “History of Medicine Sushruta – the clinician-Teacher” par Excellence (PDF) National Informatic centre, 2007.
13. Edgar J Dawlva, Elias Baydoun, Adnan Badran “Biotechnology and the developing world” Electronic Journal of Biotechnology 15(1)-fulltext-1, ISSN 0717-3458, 2002.
14. Srinivasan K “Spices as influences of body metabolism; an overview of these decades of food research International 38(1) 77-86 doi: 10; 10 fodders 2004.09.001 2005.
15. Pitter M, Abbot, NC Harkness, EF Ernst, “Location bias in controlled clinical trials of complementary/alternative therapies”. International Journal of Epidemiology 53 (5): 485-489 doi: 10.1016/s 0895-4356 (99)00220-6. PMID10812320, 2000.
16. Marciel MAM, Pinto AC, Veiga, Grynberg NF, Echevrria “A Medicinal plant , the need for multi disciplinary scientific studies” Quim Nora, 25(3): 421-38, 2002.
17. Silva NCC (1), Fernandez Junior A (1) “Biological properties of medicinal plants: a review of their antimicrobial activity”. The journal of venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199/16 (2) Pg 402-413, 2010.
18. Nascimento GGF, Locatelli J, Freitas PC, Silver GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria Braz J. Microbial 31(1): 247-250, 2000.
19. Sakagami. Y, Kanjimura K. Bactericidal activities of disinfectants against vancomycin-resistant enterococci J Hosp infec; 50(2):14, 2002.
20. Halliwell, B and Gutteridge. J. M. C. “Free radicals on Biology and medicine 3rd edition, Oxford University Press, Oxford U K, 1999.
21. Finkel.T and Holbrook, N-J Oxidants, Oxidative stress and the biology of ageing, Nature, 408,239-247, 2000.
22. Lie-Fen Shygur, Jieh-Hen Tsung, Je-Hsin Chen, Chih-Yang Chu and Chu-Ping Lo. “Antioxident properties of extracts from Medicinal plants popularly used in Taiwan” International journal of Applied Science and Engineering 3(3)195-202, 2001.
23. Park, E-J and Pezzuto, J-M “Botanicals in cancer Chemoprevention”. Cancer and Metastasis Reviews 21:-255, 2002.
24. Surh Y “Molecular mechanisms of chemopreventive effects of selected dietary and medical phenolic substances”. Mutation research, 16, and 428 (1-2): 305-27, 1999.
25. Figueroa-Hernandez J. L. Sandoval G, Jayme A.V, Figueroa-Espitea J.L, and Fernandez S.G, “Plant products with anti cancer properties employed in the treatment of Bowel cancer” Proceedings of the Western pharmacology society, 48-77-83,2005.
26. Weed H.G, Mc Gandey R and Kennedy A. “Protection against dimethyle hydrazones-induced adenomatours tumors of the mouse colon by the dietary addition of an extract of soya beans containing the Bowman-Birk protease inhibitor”. Carcinogenesis 6(8).1239 1955.
27. Kinara C, Mc Intosh GH, Record I R, and Jones GP, “Antitumor activity of extract of Zingeber aromaticum and its bioactive sesquiterpenoid Zerumbone” Nutrition and Cancer 45(2):218-25, 2003.
28. Chauhan C K, Joshi M J, Vaidya ADB, “Growth inhibition of struvite crystals in the presence of herbal extracts commiphora weights” J Master Sci; 20(1): 85-92, 2008.
29. Bensatal A, Ouahrani M R, “Inhibition of crystallization of Calcium Oxalate by the extracts of Tamarix gallica L”, Urol Res36:288-287 2008.
30. Brown K M, JR Selenium “Selenoproteins and human health: a review” Public health nutrition 4(28): 593-9 doi: 10-1079/PHN 2001 143 PMID 11663552, 2011.