Main Article Content
Dichrostachys cinerea, physicochemical, phytochemical, TLC, bioactive,
Natural products from medicinal plants, either as pure compounds or as standardized extracts, provide unlimited opportunities for new drug because of the unmatched availability of chemical diversity. Due to an increasing demand for chemical diversity, seeking therapeutic drugs from natural products interest particularly in edible plants has grown throughout the world.
Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analytical methodologies, which include the extraction, isolation and characterization of active components in leaves of Dichrostachys cinerea. The analysis of bioactive compounds present in the plant extracts involve the application of common phytochemical screening tests, physicochemical tests, chromatographic techniques such as HPLC and TLC were discussed.
2. Babu et.al., Evaluation of actue toxicity and hepatoprotective activity of the methanolic extract of Dichrostachys cinerea (Wight and Arn.) leaves, Pharmacognosy Research, 3(1), 2011, 40-43.
3. Hurinanthan, Immune modulatory effect of Dichrostachys cinerea ,Carpobrotus dimidiatus, Capparisto mentosa and Leonotis leonurus, 2009, 116-121.
4. Akindele and Adeyemi , Anti- inflammatory activity of the aqueous leaf extract of Byrsocarpuscoccineus, Fitoterapia, 78, 2007, 25-28.
5. N. K. Dubey, Rajesh Kumar and Pramila Tripathi, Global promotion of herbal medicine: India’s opportunity, Current science, 86(1), 2004, 37-41.
6. Gordon M. Cragg and David J. Newman, Natural product drug discovery in the next millennium, Journal of pharmaceutical Biology 39(1), 2001, 8-17.
7. Savithramma et.al., Screening of medicinal plants for secondary metabolites, Middle – East Journal of Scientific Research,( 8), (3), (2011), 579-584.
8. Sapna Shrikumar, T. K. Rav, Approaches towards development and promotion of herbal drugs, pharmacognosy review 1(1), 2007, 180-184.
9. Savithramma et.al., Phytochemical screening of traditional medicinal plants. Journal of Pharmacy Research 4(10), 2011, 3414-3416.
10. Mammenet.al., Variations in values of proximate analysis in Aerva lanata Juss Ex schultes, Hedyotis corymbosa (L.) LAM and Leptadenia Reticulata (Retz.)W.&A, International Journal of Pharma & Bio Science 1(4), 2010, 629-636.
11. Michel Martin and Georges Guiochon, Effects of high pressure in liquid chromatography. Journal of Chromatography 2005(1090), (1-2), 2005,16-38.
12. A. Jeena Pearl, Phytochemical studies in the leaves of Ecbolcium linneanum- The medicinal plant, International Journal of Research in Engineering and Bioscience, 1(1), 2013, 111-117.
13. Ankur Choudhary,Test for sulphated ash and Total ash. (ONLINE) Available at: htpp:\\www.Pharmaguideline.com/2011/06/test- for- sulphated ash and – total-ash.html l(Accessed on 19, Jan 2015), 2011
14. Prashant et.al.,. Phytochemical screening and Extraction: A Review, Internationale Pharmaceutica Sciencia 1(1), 2011, 98-105.
15. Vijajlakshmi and Ravindhran., Preliminary comparative and phytochemical screening of root extracts of Diospyrus ferrea (Wild.)Bakh and Aerva lanata (L) Juss. Ex Schultes. Asian Journal of Plant Science and Research 2(5), 2012, 581-587.
16. Yamunadevi Mariswamy, Wesely Edward Gnaraj, M Johnson, Chromatog raphic finger print analysis of steroids in Aerva lanata.L by HPTLC technique , Asian Journal of Tropical Biomedicine 1(6), 2011, 428-433.
17. Havesteen, Flavonoids a class of natural products for antimicrobial agents. Eur J clin Microbiol Infect Dis (9), 1990, 455- 461.
18. Harbone and Williams,. Advances in Flavonoids research since 1992. Phytochemistry (55), 2000, 481-504.
19. Shi et.al ., Saponins from edible legumes: Chemistry, processing and health benefits, J Med Food, 7(1), 2004, 67-78.