Manbir Kaur
Department of Biotechnology,, GNG College, Ludhiana, Punjab
July, 2015
 

Abstract
This work supports successful synthesis of silver nanoparticles from spinacia oleracea leaf extract by using biological approach. Spinach is selected for the isolation of silver nanoparticles because of its medicinal importance. Spinach is credited with anti-inflammatory, antioxidant, antimicrobial, antihepatic as well as anticancerous properties.

In this work spinach leaf extract is prepared by using two methods hot percolation and cold percolation. Both hot perculated and cold perculated extracts are then augmented with AgNO3 treatment and kept on different parameters like Temperature (0 degree centigrade, -20degree centigrade, 4 degree centigrade, 37 degree centigrade, 100 degree centigrade), pH (9, 10, 11), time (0min, 20min, 60min, 48hours, 72hours) and extract concentration (0.5ml, 2.5ml, 4.5ml). Then the UV-VIS spectrophotometric analysis is done of the extract suspension and it was observed that number of silver nanoparticles increases with the increase in temperature, pH, time and extract concentration. More number of silver nanoparticles are produced at temperature 100 degree centigrade, pH 11, time 72 hours and extract concentration 4.5ml. TEM analysis is done as a confirmatory test for the characterization of the silver nanoparticles.

For the TEM analysis the samples from the variants with maximum absorbance were selected that is temperature (1000C), pH (11), time (72 hours) and extract concentration (4.5ml) and from their suspension form they are converted into the pellet form and this pellet is then preserved in triple distilled water until the TEM analysis is done. Using drop method TEM images are obtained which shows mostly spherical and triangle shaped nanoparticles of 10-12nm sized. Then the isolated nanoparticles are tested for their antimicrobial activity against four clinically isolated pathogens Enterococcus, Pseudomonas aeruginasa, E.coli and Klebsella Pneumoniae by using agar well diffusion assay method. Nanoparticles show their antimicrobial activity against Enterococcus and Pseudomonas aeruginasa by forming zone of inhibition of 14mm in size.

This study opens a new way of finding ecofriendly and cheap nanoparticles from spinach leaf. More and more research on different plants for the synthesis of silver nanoparticles and their medicinal activity is under investigation in our research groups. This environmentally friendly method of biological silver nanoparticles synthesis can potentially be applied in various products that directly come in contact with the human body such as cosmetics, food and consumer goods besides medical applications.

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ISSN: 2321-2543

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